2021-04-22 21:36 Pull-down assay is a simple and sensitive in vitro approach to studying protein-protein interactions (PPIs). In methodology, pull-down assay is similar to co-immunoprecipitation. In experiments, they both use an affinity ligand to capture interacting proteins. However, there is a difference between co-immunoprecipitation and the pull-down assay. The co-immunoprecipitation captures protein complexes by using immobilized antibodies, while the pull-down assay binds interacting proteins by using a purified and tagged protein, as “bait”.
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