2021-06-27 19:46 Gel electrophoresis is an important methodology employed for protein analysis, which contains many different techniques, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing (IEF), and so on. SDS-PAGE, the most common gel electrophoresis used for proteins, provides an easy method to estimate the molecular weight of proteins, assess the complexity of the sample or the purity of preparation, and monitor the fractions obtained during chromatographic or other purification procedures. Moreover, electrophoretic patterns are required by ICH Q6B for the analysis of biopharmaceuticals.
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