2021-05-19 11:19
Pull-down assay is a simple and sensitive in vitro approach to studying protein-protein interactions (PPIs). In methodology, pull-down assay is similar to co-immunoprecipitation. In experiments, they both use an affinity ligand to capture interacting
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2021-05-19 11:19
Far-WesternBlot is a molecular biological method, which is based on the technique of Westernblot, to detect in vitro protein-protein interactions, especially for detecting interactions that do not require the native structure of the protein of int
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2021-05-19 11:19
Under physiological condition, most protein-protein interactions are transient, and happen in a very short duration, which make them difficult to be studied. Crosslinking reagents, or crosslinkers, provide the analytical solution to capture protei
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2021-05-19 11:19
Co-immunoprecipitation, Co-IP in short, is a widely applied technique to identify physiologically-relevant protein-protein interactions by utilizing target protein-specific antibodies to indirectly capture proteins that are bound to this specific tar
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2021-05-19 11:19
The aim of comprehensive peptidomics, is not only to identify and validate all endogenous peptides in a biological sample under investigation, but also to compare expression levels of the peptides of interest for specific biochemical processes. Thus,
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2021-04-22 21:38
Plant metabolomics is more and more used for understanding physiological processes like the cellular responses to various stimuli. For example, in response to sulfur deficiency, the metabolites will be readjusted. It is found that there is a strict r
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2021-04-22 21:37
Untargeted metabolomics, namely discovery metabolomics, involves the comparison of the metabolome between the control and test groups, to identify differences between their metabolite profiles which may be relevant to specific biological conditions.
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2021-04-22 21:37
Metabolomics refers to the systematic identification and quantification of the metabolome of a biological system at a specific point in time. And metabolome means the complete set of small-molecule chemicals found within a biological sample. In metab
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2021-04-22 21:36
Pull-down assay is a simple and sensitive in vitro approach to studying protein-protein interactions (PPIs). In methodology, pull-down assay is similar to co-immunoprecipitation. In experiments, they both use an affinity ligand to capture interacting
... дальше